{"id":686,"date":"2020-08-04T01:35:39","date_gmt":"2020-08-04T05:35:39","guid":{"rendered":"https:\/\/cib.udp.cl\/cms\/publicacion\/the-insulin-sensitizing-mechanism-of-myo-inositol-is-associated-with-ampk-activation-and-glut-4-expression-in-human-endometrial-cells-exposed-to-a-pcos-environment\/"},"modified":"2020-09-01T15:58:35","modified_gmt":"2020-09-01T19:58:35","slug":"the-insulin-sensitizing-mechanism-of-myo-inositol-is-associated-with-ampk-activation-and-glut-4-expression-in-human-endometrial-cells-exposed-to-a-pcos-environment","status":"publish","type":"publicacion","link":"https:\/\/cib.udp.cl\/en\/publicacion\/the-insulin-sensitizing-mechanism-of-myo-inositol-is-associated-with-ampk-activation-and-glut-4-expression-in-human-endometrial-cells-exposed-to-a-pcos-environment\/","title":{"rendered":"The insulin-sensitizing mechanism of myo-inositol is associated with AMPK activation and GLUT-4 expression in human endometrial cells exposed to a PCOS environment"},"content":{"rendered":"<p>Polycystic Ovary Syndrome (PCOS) is an endocrine-metabolic disorder characterized by hyperandrogenism and ovulatory dysfunction but also obesity and hyperinsulinemia. These characteristics induce an insulin-resistant state in tissues as endometrium, affecting its reproductive functions. Myo-inositol (MYO) is an insulin-sensitizer compound used in PCOS patients; however, its insulin-sensitizer mechanism is unclear. To understand the relationship of MYO with insulin action in endometrial cells, SMIT-1 (MYO-transporter) and MYO effect of protein-levels related to insulin pathway were evaluated. SMIT-1 was assessed in endometrial tissue from women with normal-weight, obesity, insulin-resistance and PCOS; additionally, using an in-vitro model of human endometrial cells exposed to environment resembling hyperinsulinic-obese-PCOS, MYO effect was evaluated on p-AMPK and GLUT-4 levels and glucose uptake by western blot, immunocytochemistry and confocal microscopy, respectively. SMIT-1 was detected in endometrial tissue from all groups and decreased in PCOS and obesity (p&lt;0.05 vs normal-weight). In in-vitro model, PCOS conditions decreased p-AMPK levels being restored with MYO (p&lt;0.05). The diminished GLUT-4 protein levels promoted by PCOS environment were restored by MYO through SMIT-1 and p-AMPK-dependent mechanism (p&lt;0.05). Also, MYO restored glucose uptake in cells under PCOS condition through a p-AMPK dependent mechanism. Finally, these results were similar to those obtained with metformin treatment in the same in-vitro conditions. Consequently, MYO could be a potential insulin sensitizer by its positive effects in insulin-resistant tissues as PCOS-endometrium, acting through SMIT-1, provoking AMPK activation and elevated GLUT-4 levels and consequently, increasing glucose uptake by human endometrial cells. Therefore, MYO may be used as an effective option in insulin-resistant PCOS women.<\/p>\n","protected":false},"featured_media":896,"template":"","class_list":["post-686","publicacion","type-publicacion","status-publish","has-post-thumbnail","hentry","revista-american-journal-of-physiology-endocrinology-and-metabolism"],"acf":[],"_links":{"self":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/publicacion\/686","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/publicacion"}],"about":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/types\/publicacion"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/media\/896"}],"wp:attachment":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/media?parent=686"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}