{"id":693,"date":"2020-08-04T01:35:39","date_gmt":"2020-08-04T05:35:39","guid":{"rendered":"https:\/\/cib.udp.cl\/cms\/publicacion\/prolactin-gene-expression-in-the-pituitary-of-rats-subjected-to-vaginocervical-stimulation-requires-erk-1-2-signaling\/"},"modified":"2020-09-01T15:55:44","modified_gmt":"2020-09-01T19:55:44","slug":"prolactin-gene-expression-in-the-pituitary-of-rats-subjected-to-vaginocervical-stimulation-requires-erk-1-2-signaling","status":"publish","type":"publicacion","link":"https:\/\/cib.udp.cl\/en\/publicacion\/prolactin-gene-expression-in-the-pituitary-of-rats-subjected-to-vaginocervical-stimulation-requires-erk-1-2-signaling\/","title":{"rendered":"Prolactin gene expression in the pituitary of rats subjected to vaginocervical stimulation requires Erk-1\/2 signaling"},"content":{"rendered":"<p>Vaginocervical stimulation (VCS) induces twice-daily prolactin (PRL) surges resulting in pseudopregnancy in the rat. Furthermore, activation of the extracellular signal-regulated kinase-1\/2 (Erk-1\/2) is involved in the effect of estradiol (E2) on the Prl gene expression in pituitary cells. Herein, we investigated whether Erk-1\/2 signaling is involved in the control of Prl expression in the pituitary of VCS rats and whether VCS regulates the effect of E2 on Erk-1\/2 and Prl in the pituitary. Estrous rats were assigned as control or VCS groups and 0, 6, 12 or 24 h later the levels and localization of phosphorylated Erk-1\/2 (p-Erk-1\/2) were analyzed in the pituitary. The effect of an Erk-1\/2 inhibitor PD98059 on the Prl level in the pituitary of control or VCS rats was also analyzed. Other control or VCS rats were treated with E2 and the level of p-Erk-1\/2 and Prl were measured in the pituitary. In control rats, p-Erk-1\/2 decreased at 6 and 12 h and increased at 24 h while Erk-1\/2 was phosphorylated at all time points in VCS rats. p-Erk-1\/2 was localized only in the anterior pituitary. PD98059 decreased Prl level in VCS, but not in control rats. Estradiol decreased Erk-1\/2 phosphorylation although did not change Prl level in the pituitary of control or VCS rats. These findings show that prolonged activation of Erk-1\/2 is necessary to induce Prl expression in the pituitary of VCS rats; however, VCS does not influence the role of E2 on the activation of Erk-1\/2 and Prl expression the pituitary.<\/p>\n","protected":false},"featured_media":896,"template":"","class_list":["post-693","publicacion","type-publicacion","status-publish","has-post-thumbnail","hentry","revista-reproductive-biology"],"acf":[],"_links":{"self":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/publicacion\/693","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/publicacion"}],"about":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/types\/publicacion"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/media\/896"}],"wp:attachment":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/media?parent=693"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}