{"id":694,"date":"2020-08-04T01:35:39","date_gmt":"2020-08-04T05:35:39","guid":{"rendered":"https:\/\/cib.udp.cl\/cms\/publicacion\/proinflammatory-environment-and-role-of-tnf-%ce%b1-in-endometrial-function-of-obese-women-having-polycystic-ovarian-syndrome\/"},"modified":"2020-09-01T15:55:25","modified_gmt":"2020-09-01T19:55:25","slug":"proinflammatory-environment-and-role-of-tnf-%ce%b1-in-endometrial-function-of-obese-women-having-polycystic-ovarian-syndrome","status":"publish","type":"publicacion","link":"https:\/\/cib.udp.cl\/en\/publicacion\/proinflammatory-environment-and-role-of-tnf-%ce%b1-in-endometrial-function-of-obese-women-having-polycystic-ovarian-syndrome\/","title":{"rendered":"Proinflammatory environment and role of TNF-\u03b1 in endometrial function of obese women having polycystic ovarian syndrome"},"content":{"rendered":"<p>Background\/objectives:\u00a0A high percentage of women having polycystic ovarian syndrome (PCOS) exhibit hyperinsulinemia and obesity. Transforming necrosis factor-\u03b1 (TNF-\u03b1) is an adipokine that increases in obesity and negatively affects insulin action in several tissues, including the endometrium. In fact, it has been reported that insulin signaling is altered in the endometrium of PCOS women, affecting its reproductive function. The aim of this study was to determine the proinflammatory environment and TNF-\u03b1 signaling in endometrium from obese women with PCOS, and also to evaluate the effect of TNF-\u03b1 on endometrial cell energy homeostasis. Methods:\u00a0Serum and endometrial tissues were obtained from four study groups: normal-weight, normal-weight-PCOS, obese and obese-PCOS (hyperandrogenemia\/hyperinsulinemia) (n=7 per group). Serum TNF-\u03b1 level was assayed by enzyme-linked immunosorbent assay (ELISA); endometrial TNF-\u03b1 level and its receptors (TNFR1\/TNFR2) as well as nuclear factor (NF)-\u03baB content were determined by immunohistochemistry. Finally, we evaluated TNF-\u03b1 effect on glucose uptake in cultured human endometrial stromal cells (T-HESC) treated or not with testosterone\/insulin resembling partially the PCOS condition. Results:\u00a0TNF-\u03b1 plasma levels were similar between groups, whereas cytokine levels and macrophage number increased in endometrium from obese-PCOS women (P&lt;0.001). Both receptor types were higher in obese vs normal-weight women, particularly TNFR2 content in the obese-PCOS group (P&lt;0.001). Furthermore, an increased NF-\u03baB nuclear content in endometrium from obese-PCOS was observed (P&lt;0.001). Finally, TNF-\u03b1 treatment of T-HESC cultures exhibited a decrease of glucose uptake (P&lt;0.05), although similar to cells treated with testosterone or testosterone\/insulin\/TNF-\u03b1. Conclusions:\u00a0These results suggest that the PCOS condition induces an inflammatory state exacerbated when obesity is present, where a higher TNF-\u03b1 signaling is observed, all of which could affect glucose uptake in the tissue and may cause fertility failures in these women.<\/p>\n","protected":false},"featured_media":896,"template":"","class_list":["post-694","publicacion","type-publicacion","status-publish","has-post-thumbnail","hentry","revista-international-journal-of-obesity"],"acf":[],"_links":{"self":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/publicacion\/694","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/publicacion"}],"about":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/types\/publicacion"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/media\/896"}],"wp:attachment":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/media?parent=694"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}