{"id":699,"date":"2020-08-04T01:35:39","date_gmt":"2020-08-04T05:35:39","guid":{"rendered":"https:\/\/cib.udp.cl\/cms\/publicacion\/mating-decreases-plasma-levels-of-tgfb1-and-regulates-myosalpinx-expression-of-tgfb1-tgfbr3-in-the-rat\/"},"modified":"2020-09-01T15:54:07","modified_gmt":"2020-09-01T19:54:07","slug":"mating-decreases-plasma-levels-of-tgfb1-and-regulates-myosalpinx-expression-of-tgfb1-tgfbr3-in-the-rat","status":"publish","type":"publicacion","link":"https:\/\/cib.udp.cl\/en\/publicacion\/mating-decreases-plasma-levels-of-tgfb1-and-regulates-myosalpinx-expression-of-tgfb1-tgfbr3-in-the-rat\/","title":{"rendered":"Mating decreases plasma levels of TGFb1 and regulates myosalpinx expression of TGFb1\/TGFBR3 in the rat"},"content":{"rendered":"<p>Mating shuts down a 2-methoxyestradiol (2ME)-dependent, non-genomic activity that is responsible for accelerating egg transport in the rat oviduct. The aims of this work were to investigate the role of TGF\u03b21 in this 2ME-reduced activity and to determine the effect of mating on the expression and distribution of TGF\u03b21 and its receptor TGFBR3 in the rat oviduct. We determined the level of TGF\u03b21 in the plasma and oviductal fluid at 1, 3, or 6 hr during Day 1 of the oestrous cycle in unmated or mated animals. We then examined if 2ME accelerates oviductal egg transport in unmated rats that were previously treated with a neutralizing TGF\u03b21 antibody. The expression of Tgfb1 and Tgfbr3 mRNA and the level and distribution of TGFBR3 protein in the oviduct were also determined at these time points. Mating decreased TGF\u03b21 in the plasma, but not in the oviductal fluid, whereas antibody neutralization of circulating TGF\u03b21 did not prevent the effect of 2ME on egg transport. Mating decreased Tgfb1 and hastened the increase in TGFBR3 abundance in the myosalpinx. These results indicate that mating decreased circulating levels of TGF\u03b21 without shutting down the non-genomic 2ME response that normally accelerates egg transport. Levels of Tgfb1 transcript and TGFBR3 protein, however, changed in the myosalpinx of mated rats, suggesting a role for mating-associated factors in the autocrine and paracrine effects of TGF\u03b2 in the oviduct.<\/p>\n","protected":false},"featured_media":896,"template":"","class_list":["post-699","publicacion","type-publicacion","status-publish","has-post-thumbnail","hentry","revista-molecular-reproduction-and-development"],"acf":[],"_links":{"self":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/publicacion\/699","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/publicacion"}],"about":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/types\/publicacion"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/media\/896"}],"wp:attachment":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/media?parent=699"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}