{"id":702,"date":"2020-08-04T01:35:39","date_gmt":"2020-08-04T05:35:39","guid":{"rendered":"https:\/\/cib.udp.cl\/cms\/publicacion\/tumour-necrosis-factor-%ce%b1-is-the-signal-induced-by-mating-to-shutdown-a-2-methoxyestradiol-nongenomic-action-necessary-to-accelerate-oviductal-egg-transport-in-the-rat\/"},"modified":"2020-09-01T15:53:23","modified_gmt":"2020-09-01T19:53:23","slug":"tumour-necrosis-factor-%ce%b1-is-the-signal-induced-by-mating-to-shutdown-a-2-methoxyestradiol-nongenomic-action-necessary-to-accelerate-oviductal-egg-transport-in-the-rat","status":"publish","type":"publicacion","link":"https:\/\/cib.udp.cl\/en\/publicacion\/tumour-necrosis-factor-%ce%b1-is-the-signal-induced-by-mating-to-shutdown-a-2-methoxyestradiol-nongenomic-action-necessary-to-accelerate-oviductal-egg-transport-in-the-rat\/","title":{"rendered":"Tumour necrosis factor-\u03b1 is the signal induced by mating to shutdown a 2-methoxyestradiol nongenomic action necessary to accelerate oviductal egg transport in the rat."},"content":{"rendered":"<p>Mating shut down a 2-methoxyestradiol (2ME) nongenomic action necessary to accelerate egg transport in the rat oviduct. Herein, we investigated whether tumour necrosis factor-\u03b1 (TNF-\u03b1) participates in this mating effect. In unmated and mated rats, we determined the concentration of TNF-\u03b1 in the oviductal fluid and the level of the mRNA for Tnf-a (Tnf) and their receptors Tnfrsf1a and Tnfrsf1b in the oviduct tissues. The distribution of the TNFRSF1A and TNFRSF1B proteins in the oviduct of unmated and mated was also assessed. Finally, we examined whether 2ME accelerates oviductal egg transport in unmated rats that were previously treated with a rat recombinant TNF-\u03b1 alone or concomitant with a selective inhibitor of the NF-\u03baB activity. Mating increased TNF-\u03b1 in the oviductal fluid, but Tnf transcript was not detected in the oviduct. The mRNA for TNF-\u03b1 receptors as well as their distribution was not affected by mating, although they were mainly localized in the endosalpinx. Administration of TNF-\u03b1 into the oviduct of unmated rats prevented the effect of 2ME on egg transport. However, the NF-\u03baB activity inhibitor did not revert this effect of TNF-\u03b1. These results indicate that mating increased TNF-\u03b1 in the oviductal fluid, although this not associated with changes in the expression and localization of TNF-\u03b1 receptors in the oviductal cells. Furthermore, TNF-\u03b1 mimicked the effect of mating on the 2ME-induced egg transport acceleration, independently of the activation of NF-\u03baB in the oviduct. We concluded that TNF-\u03b1 is the signal induced by mating to shut down a 2ME nongenomic action in the rat oviduct.<\/p>\n","protected":false},"featured_media":896,"template":"","class_list":["post-702","publicacion","type-publicacion","status-publish","has-post-thumbnail","hentry","revista-reproduction"],"acf":[],"_links":{"self":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/publicacion\/702","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/publicacion"}],"about":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/types\/publicacion"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/media\/896"}],"wp:attachment":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/media?parent=702"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}