{"id":704,"date":"2020-08-04T01:35:39","date_gmt":"2020-08-04T05:35:39","guid":{"rendered":"https:\/\/cib.udp.cl\/cms\/publicacion\/tnf-alpha-and-adiponectin-pathways-are-deregulated-in-endometria-from-obese-women-with-polycystic-ovarian-syndrome\/"},"modified":"2020-09-01T15:52:54","modified_gmt":"2020-09-01T19:52:54","slug":"tnf-alpha-and-adiponectin-pathways-are-deregulated-in-endometria-from-obese-women-with-polycystic-ovarian-syndrome","status":"publish","type":"publicacion","link":"https:\/\/cib.udp.cl\/en\/publicacion\/tnf-alpha-and-adiponectin-pathways-are-deregulated-in-endometria-from-obese-women-with-polycystic-ovarian-syndrome\/","title":{"rendered":"TNF-[alpha] and adiponectin pathways are deregulated in endometria from obese women with polycystic ovarian syndrome."},"content":{"rendered":"<p>Seventy percent of women bearing PCOS are obese; adiponectin and TNF\u03b1, as obesity markers, have a dual role in the sensitivity and action of insulin. Adiponectin (insulin sensitising) decreases, whereas, TNF\u03b1, IL6 (negative regulators of insulin pathway) increases in obese-women. Moreover, TNF\u03b1 decreases the transcript and protein levels of adiponectin. These changes could affect the normal energetic status in endometrium, tissue that exhibits abnormal insulin signalling in the PCOS condition (hyperandrogenic\/hyperinsulinic environment). The aim of the present work was to evaluate molecules involved in TNF\u03b1 (RTNF\u03b1 1 y 2, NFkB, p-IKK) and adiponectin (AdipoR1, AdipoR2, APPL1, TAK1, MEK1) signalling in endometria (n=15) from lean, Obese and Obese-PCOS women by western-blot, immunohistochemistry and real-time PCR. Also, the number of macrophages was evaluated by CD68 and plasma levels of adiponectin, TNF\u03b1 and IL-6 were assayed by ELISA. No changes in serum TNF-\u03b1 levels were observed, however, IL-6 levels increase in both Obese groups vs Lean (P&lt;0.01). Adiponectin was lower in Obese-SOP (P&lt;0.01). TNF\u03b1, its receptors and CD68 levels increase in Obese-PCOS vs other groups (P&lt;0.05). Transcripts levels for adiponectin, TNF\u03b1\/receptors and APPL1 were similar in the three groups of endometria. NFkB nuclear localization was higher in Obese-SOP vs the other groups (P&lt;0.05) and activated IKK increased markedly in both groups of Obese vs Lean group (P&lt;0.0001). Adiponectin protein levels (plasma and endometrial), AdipoR1, APPL1 and MEK1 were low in Obese-PCOS vs Lean group (P&lt;0.05); TAK1 diminished in both Obese endometria (P&lt;0.05). Consequently, there is an increased inflammatory environment in endometrium of Obese-PCOS women that could decrease adiponectin signalling, through the participation of TNF\u03b1, NF\u03baB activation and\/or IL-6 signalling; therefore, affecting insulin signalling under obesity, hyperandrogenic and hyperinsulinic conditions, compromising the energetic metabolism for normal endometrial function.<\/p>\n","protected":false},"featured_media":896,"template":"","class_list":["post-704","publicacion","type-publicacion","status-publish","has-post-thumbnail","hentry","revista-endocrine-abstracts"],"acf":[],"_links":{"self":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/publicacion\/704","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/publicacion"}],"about":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/types\/publicacion"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/media\/896"}],"wp:attachment":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/media?parent=704"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}