{"id":739,"date":"2020-08-04T01:35:41","date_gmt":"2020-08-04T05:35:41","guid":{"rendered":"https:\/\/cib.udp.cl\/cms\/publicacion\/behavioral-effects-of-manganese-injected-in-the-rat-substantia-nigra-are-potentiated-by-dicumarol-a-dt-diaphorase-inhibitor\/"},"modified":"2020-09-05T12:21:48","modified_gmt":"2020-09-05T16:21:48","slug":"behavioral-effects-of-manganese-injected-in-the-rat-substantia-nigra-are-potentiated-by-dicumarol-a-dt-diaphorase-inhibitor","status":"publish","type":"publicacion","link":"https:\/\/cib.udp.cl\/en\/publicacion\/behavioral-effects-of-manganese-injected-in-the-rat-substantia-nigra-are-potentiated-by-dicumarol-a-dt-diaphorase-inhibitor\/","title":{"rendered":"Behavioral effects of manganese injected in the rat substantia nigra are potentiated by dicumarol, a DT-diaphorase inhibitor"},"content":{"rendered":"<p>The purpose of this study was to evaluate the contribution of DT-diaphorase inhibition to in vivo neurodegenerative effects of dopamine (DA) oxidation to the corresponding o-quinones. The neurotoxicity to nigrostriatal DA neurons was induced by injection of manganese pyrophosphate (Mn(3+)) complex as a prooxidizing agent alone or together with the DT-diaphorase inhibitor dicumarol into the right rat substantia nigra. The behavioral effects were compared with those induced after selective lesions of dopaminergic neurons with 6-hydroxydopamine (6-OHDA). Intranigral injection of Mn(3+) and Mn(3+) plus dicumarol produced significant impairment in motor behavior compared with control animals. However, the effect seen in the Mn(3+) plus dicumarol injected group was significantly more severe than that observed in the Mn(3+) alone injected group. In motor activity and rearing behavior, the simultaneous injection of Mn(3+) plus dicumarol produced a 6-OHDA-like impairment. Similar effects were observed in the acquisition of a conditioned avoidance response (CAR). Dicumarol significantly impaired avoidance conditioning although without affecting the motor behavior. The behavioral effects were correlated to the extent of striatal tyrosine hydroxylase (TH)-positive fiber loss. Rats receiving unilateral intranigral Mn(3+) and Mn(3+) plus dicumarol injections exhibited a significant reduction in nigrostriatal TH-positive fiber density in medial forebrain bundle compared with the contralateral noninjected side. In conclusion, this study provides evidence that the neurotoxicity of Mn(3+) in vivo is potentiated by DT-diaphorase inhibition, suggesting that this enzyme could play a neuroprotective role in the nigrostriatal DA systems.<\/p>","protected":false},"featured_media":907,"template":"","class_list":["post-739","publicacion","type-publicacion","status-publish","has-post-thumbnail","hentry","lineas-neuropatologia","revista-pharmacology-biochemistry-and-behavior"],"acf":[],"_links":{"self":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/publicacion\/739","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/publicacion"}],"about":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/types\/publicacion"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/media\/907"}],"wp:attachment":[{"href":"https:\/\/cib.udp.cl\/en\/wp-json\/wp\/v2\/media?parent=739"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}